trpv1 channels human visceral adipose tissue  (Alomone Labs)

Journal: Circulation Research

Article Title: Activation of Transient Receptor Potential Vanilloid Type-1 Channel Prevents Adipogenesis and Obesity

doi: 10.1161/01.res.0000262653.84850.8b

Figure Lengend Snippet: Figure 2. Reduced TRPV1 expression in visceral adipose tissue from obese db/db mice (upper panel), obese ob/ob mice (middle panel) and obese human male subjects (lower panel). Picture of body shape and abdominal situs showing visceral fat (a and e); expres- sion of TRPV1 in visceral adipose tissue (b and f); representative agarose gel electrophoresis of PCR products from mRNA of TRPV1 and GAPDH genes in visceral adipose tissue (c and g), M denotes bp marker; and summary data (d and h); each n4. **P0.01 between groups. Lower panels show data from age-matched human male control subjects and obese human male subjects. Waist cir- cumference (i); each n8. ***P0.001 between groups; expression of TRPV1 in visceral adipose tissue and subcutaneous fat (j); repre- sentative quantitative real-time RT-PCR from visceral adipose tissue (k) from 1 human male control subject (black line) and from 1 obese human male subject (red line); y-axis denotes fluorescence at 530 nm in arbitrary units, and x-axis denotes number of cycles of the quantitative real-time RT-PCR; The shift of the red line to the right compared with the black line indicates reduced TRPV1 tran- scripts in obese subjects; summary data of quantitative real-time RT-PCR are given in (l); n4. *P0.05 between groups. Quantitative in-cell Western assay of TRPV1 expression in visceral adipose tissue (m), representative capsaicin-induced calcium influx into visceral adipose tissue from 1 human control subject (open circles) and 1 obese human subject (filled circles) (n); and summary data (o); each n4; *P0.05).

Article Snippet: In-cell Western assay of TRPV1 channels human visceral adipose tissue was performed using the Odyssey infrared imaging system (Licor biosciences) as described by our group.9 Primary rabbit anti- human TRPV1-antibodies were obtained from Alomone labs (Jerusalem, Israel) and secondary IRDye800CW-infrared fluorescent dye-conjugated goat anti-rabbit antibodies were obtained from Biomol (Hamburg, Germany).

Techniques: Expressing, Agarose Gel Electrophoresis, Marker, Control, Quantitative RT-PCR, In-Cell ELISA

Journal: Circulation Research

Article Title: Activation of Transient Receptor Potential Vanilloid Type-1 Channel Prevents Adipogenesis and Obesity

doi: 10.1161/01.res.0000262653.84850.8b

Figure Lengend Snippet: Figure 3. The TRPV1 channel agonist capsaicin prevents obesity in mice on high fat diet. a, Time course of body weight from control mice (open circles), mice on high fat diet (filled circles), control mice with capsaicin (open squares), and mice on high fat diet with cap- saicin (filled squares; each n10); Body weight (b), daily food intake per mouse during the first 10 days after the start of capsaicin administration in control mice (open circles), mice on high fat diet (filled circles), control mice with capsaicin (open squares), and mice on high fat diet with capsaicin (filled squares; each n10) (c), picture of body shape of mice (d), waist circumference (e), serum triglyc- erides (f), visceral adipose tissue (g), subcutaneous fat (h), brown fat (i) at the age of 120 days of control mice (n10), mice on high fat diet (n10), control mice plus capsaicin (n10), and mice on high fat diet plus capsaicin (n10). **P0.01 for the comparison high fat vs control; P0.01 for the comparison high fat plus capsaicin vs high fat.

Article Snippet: In-cell Western assay of TRPV1 channels human visceral adipose tissue was performed using the Odyssey infrared imaging system (Licor biosciences) as described by our group.9 Primary rabbit anti- human TRPV1-antibodies were obtained from Alomone labs (Jerusalem, Israel) and secondary IRDye800CW-infrared fluorescent dye-conjugated goat anti-rabbit antibodies were obtained from Biomol (Hamburg, Germany).

Techniques: Control, Comparison

Journal: Circulation Research

Article Title: Activation of Transient Receptor Potential Vanilloid Type-1 Channel Prevents Adipogenesis and Obesity

doi: 10.1161/01.res.0000262653.84850.8b

Figure Lengend Snippet: Figure 4. The TRPV1 channel agonist capsaicin prevents adipocyte hypertro- phy and the reduction of TRPV1 expres- sion in visceral adipose tissue from mice on high fat diet. a, Representative cross sections of visceral adipose tissue (vis fat), subcutaneous fat (sc fat), and brown fat (br fat) from control mice, mice on high fat diet, control mice plus capsaicin, and mice on high fat diet plus capsaicin. Scale bar indicates 10 m; magnifica- tion, 400. Summary data of adipocyte size of visceral adipose tissue (b), subcu- taneous fat (c), and brown fat (d) in mice. ***P0.001 for the comparison high fat vs control; P0.001 for the com- parison high fat plus capsaicin vs high fat. e, Expression of TRPV1 in visceral adipose tissue (vis fat) from mice. ***P0.001 for the comparison high fat vs control; P0.001 for the com- parison high fat plus capsaicin vs high fat. f, Representative agarose gel electro- phoresis of PCR products from mRNA of TRPV1 and GAPDH genes in visceral adipose tissue mice M denotes bp marker. Panels are representative of 3 separate experiments showing similar results.

Article Snippet: In-cell Western assay of TRPV1 channels human visceral adipose tissue was performed using the Odyssey infrared imaging system (Licor biosciences) as described by our group.9 Primary rabbit anti- human TRPV1-antibodies were obtained from Alomone labs (Jerusalem, Israel) and secondary IRDye800CW-infrared fluorescent dye-conjugated goat anti-rabbit antibodies were obtained from Biomol (Hamburg, Germany).

Techniques: Control, Comparison, Expressing, Agarose Gel Electrophoresis, Marker